https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:28948 VDR) polymorphisms, BsmI and ApaI. Methods: Two hundred participants completed food frequency and supplement questionnaires, and were assayed for circulating let-7b expression by qPCR. Polymorphisms were detected using restriction fragment length polymorphism-PCR. Results: let-7b expression negatively correlated with vitamin D intake (r_s = -0.20, p = 0.005). The magnitude and direction of correlation were maintained in the presence of the BsmI restriction site (r_s = -0.27, p = 0.0005). However, in the absence of BsmI restriction site, the direction of the correlation was reversed (r_s = +0.319, p = 0.0497). These correlations were significantly different (z-score = 2.64, p = 0.0085). The correlation between vitamin D intake and let-7a was only significant in those without the ApaI restriction site. Conclusions: The correlation between vitamin D intake and let-7a/b expression in this cohort varies with VDR genotype. This study highlights the importance of considering underlying genotypic variance in miRNA expression studies and in nutritional epigenetics generally.]]> Wed 11 Apr 2018 14:17:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:6997 T-MTHFR, 1298A>C-MTHFR, 80G>A-RFC, 2756A>G-MS, 66A>G- MSR, 19bpDHFR and 1561C>T-GCPII), only 677C>T-MTHFR was a significant risk for hypertension: OR 1.89; 95% CI 1.07–3.32 (χ² p = 0.038). Additionally, hypertensive subjects had a significantly lower intake of dietary folate than normotensive individuals (p = 0.0221), although this did not markedly alter blood metabolite levels. Several significant linear associations between dietary folate and related blood metabolites were found in normotensive subjects (p<0.001 for Hcy, red cell and serum folate) and were as predicted on an a priori basis – generally weaker associations existed in hypertensive subjects (p<0.05 for serum folate). This was true for data examined collectively or by genotype. Multiple regression analysis for diastolic or systolic blood pressure showed significant interaction for gender and folate intake (p = 0.014 and 0.019, respectively). In both cases this interaction occurred only in females, with higher folate intake associated with decreased blood pressure. Regressing diastolic blood pressure and 677C>T-MTHFR genotype showed significance (males; p = 0.032) and borderline significance (all subjects). Conclusion: Dietary folate and 677C>T-MTHFR genotype may modify blood pressure.]]> Sat 24 Mar 2018 08:37:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4945 Sat 24 Mar 2018 07:48:07 AEDT ]]>